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| Registros recuperados : 1 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
02/01/2017 |
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Data da última atualização: |
20/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MOREIRA, C. M.; ANDRADE, H. B. de; BERTOLUCCI, S. K. V.; LAMEIRA, O. A.; MOHAMMED, A.; PINTO, J. E. B. P. |
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Afiliação: |
Carolina Mariane MOREIRA, UFLA; Helena Botelho de ANDRADE, UFLA; Suzan Kelly Vilela BERTOLUCCI, UFLA; OSMAR ALVES LAMEIRA, CPATU; Aliyu MOHAMMED, Abant ?zzet Baysal University; José Eduardo Brasil Pereira PINTO, UFLA. |
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Título: |
Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Turkish Journal of Biology, v. 40, n. 6, p. 1227-1234, 2016. |
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DOI: |
10.3906/biy-1601-56 |
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Idioma: |
Inglês |
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Conteúdo: |
The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6- trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%. |
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Palavras-Chave: |
Regeneração in vitro. |
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Thesagro: |
Curaua; Regeneração. |
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Thesaurus NAL: |
thidiazuron. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152619/1/Carolina-Moreira-Plantlet-regeneration-curaua-2016.pdf
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Marc: |
LEADER 02130naa a2200241 a 4500
001 2059742
005 2022-05-20
008 2016 bl uuuu u00u1 u #d
024 7 $a10.3906/biy-1601-56$2DOI
100 1 $aMOREIRA, C. M.
245 $aPlantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species.$h[electronic resource]
260 $c2016
520 $aThe present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6- trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%.
650 $athidiazuron
650 $aCuraua
650 $aRegeneração
653 $aRegeneração in vitro
700 1 $aANDRADE, H. B. de
700 1 $aBERTOLUCCI, S. K. V.
700 1 $aLAMEIRA, O. A.
700 1 $aMOHAMMED, A.
700 1 $aPINTO, J. E. B. P.
773 $tTurkish Journal of Biology$gv. 40, n. 6, p. 1227-1234, 2016.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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